端粒调控因子tankyrase在胃腺癌中的表达

Expression of telomere regulating factor tankyrase in gastric adenocarcinoma

目的:研究端粒调控因子tankyrase在胃腺癌组织中的表达及其在胃腺癌发生和发展过程中的作用。方法:应用荧光实时定量PCR(Real-timePCR)技术检测16例胃腺癌组织及离肿瘤边缘5cm处的正常癌旁组织中tankyrasemRNA表达水平。应用Envision免疫组化法检测37例胃腺癌和13例正常对照组织中tankyrase蛋白质表达水平。分析tankyrase表达水平在胃腺癌临床分期和组织分化程度各组间的关系。结果:胃腺癌中tankyrasemRNA的表达[1.44×10-2,(3.88×10-5)-0.4847]高于正常对照[1.0134×10-2,0-(4.933×10-2)](P<0.05)。胃腺癌中tankyrase蛋白质表达高于正常对照(P<0.05)。但在不同胃腺癌临床分期、组织分化程度各组之间均未见统计学差异。结论:TankyrasemRNA和蛋白水平在胃腺癌中的表达明显增高,可能是胃腺癌发生过程中的重要因素。

AIM： To detect the expression level of telomere regulating factor tankyrase and its clinical significhnce in the origin and development of gastric adenocarcinoma. METHODS： Quantitative Real - time PCR was used to measure the expression of tankyrase mRNA in cancerous and normal adjacent tissues from 16 patients with gastric adenocarcinoma. Immunohistocbemistry was used to detect tankyrase protein expressions in 37 gastric adenocarcinoma samples and 13 normal controls. RESULTS： The expression of tankyrase mRNA in gastric adenocaecinoma [ median 1. 44 ×10^-2, range （3. 88 ×10^-5 ） -0. 4847] was significantly higher than that in normal adjacent mucosa [ median 1. 0134 ×10^-2, range 0 - （4. 933 ×10^-2） ] （ P〈0. 05 ）. The protein expression level of tankyrase in gastric adenocarcinomas was significantly higher than that in normal controls （P 〈 0. 05 ）. No significant difference was observed between different clinical stages and different pathological grades. CONCLUSION： Tankyrase was overexpressed in gastric adenocarcinomas. It may become one of the markers for detecting gastric adenocarcinomas.