威麦宁对小鼠Lewis肺癌细胞周期的影响(英文)

Influence of Weimaining on the cell cycle of murine Lewis lung carcinoma

目的:探讨威麦宁对小鼠Lewis肺癌的抑制作用,并检测其对细胞周期的影响。方法:采用流式细胞术分析、免疫组化法检测药物对模型鼠肿瘤细胞周期及对cyclinD1表达的影响。结果:威麦宁浓度在100mg·kg-1·d-1、150mg·kg-1·d-1、200mg·kg-1·d-1、250mg·kg-1·d-1时,对小鼠Lewis肺癌的抑制率分别为19.14%、33.59%、40.63%、51.56%,药物对肿瘤的抑制作用具有剂量依赖性;该药物可将肿瘤细胞阻滞在G0-G1期,并降低肿瘤细胞cyclinD1的表达(P<0.01)。结论:威麦宁对小鼠Lewis肺癌抑制作用的机制之一可能是通过降低肿瘤细胞cyclinD1的表达,将肿瘤细胞阻止于G0-G1期,使细胞不能进入S期进行DNA复制,从而抑制肿瘤的生长。

AIM： To investigate the anti -tumor effect of Weimaining （WMN） on a murine Lewis lung carcinoma （3LL） and the influence on the cell cycle. METHODS： The inhibitory rate of WMN in 3LL growth was detected by replicating the model of 3LL. The effect of the drug on 3LL cell cycle and the influence of the drug on the expression of cyclin D1 protein were investigated by flow cytometry and immunohistochemical staining. RESULTS ： The results showed that the inhibitory rate of drug in 3LL is 19. 14%, 33.59%, 40. 63% and 51.56% respectively at dosage ranging from 100, 150, 200 and 250 mg·kg^-1·d^-1. The drug inhibits tumor growth in a dose-dependent manner. The drug arrests 3LL cells in Go -G1 phase and decreases the expression of cyclin D1 protein. CONCLUSION： WMN inhibits the growth of 3LL cells in vivo by decreasing the expression of cyclin D1, blocking the cells in Go - G1 phase and preventing the cells transition from G1 to S phase while DNA is replicated.