非何杰金淋巴瘤p15基因操纵区5’-CpG岛的甲基化的研究

5’-GpG islands of p15 gene hypermethlation in non-Hodgkin’s lymphomas

目的:探索p15基因异常甲基化在非何杰金淋巴瘤(NHL)中的变化及其在各分型中的表达。方法:用特异PCR(MSP)的方法检测32例非何杰金淋巴瘤石蜡标本p15基因甲基化;检测非何杰金淋巴瘤患者标本21例,用RT-PCR方法测定p15基因的表达。结果:非何杰金淋巴瘤p15基因操纵区甲基化的发生率为18.9%(10/53),高度恶性比低度恶性更易发生甲基化,其发生率分别为27.3%和0%。结论:p15基因甲基化可能是非何杰金淋巴瘤发病的原因之一,并与病程进展有关。

AIM： To illustrate the expression of hypermethylation p15 gene in 53 non - Hodgkin＇ s lymphoma （NHL）. METHODS： The methylation ofpl5 gene in 53 cases of non - Hodgkin＇ s lymphoma was detected by using methylation- specific PCR （MSP） technique. RESULTS： 18.9% （10/53） in NHL were methylation in p15 gene. p15 gene was frequently in high malignant NHL patients （27.3%） compared with in low malignant patients （0%）. CONCLUSION： The result suggested that the hypermethylation of p15 may play an important role in non - Hodgkin＇ s lymphoma.