大鼠神经元Arnt2亚细胞定位的预测与分析

Prediction and analysis of the subcellular localization of Arnt2 in rat cerebellar granule neurons

目的:分析大鼠小脑颗粒神经元Arnt2的亚细胞定位情况。方法:利用CBS生物信息资源,根据Arnt2氨基酸序列(LOCUS:NP_036913)进行真核生物亚细胞定位预测,并寻找Arnt2核输出信号(NES);最后利用激光扫描共聚焦显微术(LSM)确定Arnt2在正常大鼠小脑颗粒神经元中的亚细胞定位情况。结果:预测Arnt2在真核生物细胞中主要为细胞核定位,并具有线粒体定位的可能性;预测结果还显示Arnt2氨基酸序列上存在着核输出信号,具体位置为氨基端第143位亮氨酸;LSM分析结果证实Arnt2定位于大鼠小脑颗粒神经元细胞核内。结论:Arnt2定位于正常大鼠小脑颗粒神经元细胞核内;Arnt2具有线粒体定位的可能性,并存在核输出信号,提示在某些诱导因素作用下,Arnt2存在线粒体定向转位的趋势。

AIM： To analyze the subcellular localization of Arnt2 in rat cerebellar granule neurons （CGNs）. METHODS： Based on the amino acids sequence of Arnt2 （LOCUS： NP_036913 ）, the subeellular localization of Arnt2 in eukaryotic cells and the nuclear export signals （NES） of Arnt2 were predicted in CBS bioinformatics database. The subcellufar localization of Arnt2 in rat cerebellar granule neurons was detected by the method of laser scanning confocal microscopy （LSM） analysis. RESULTS： It was predicted that Arnt2 located in nuclei of eukaryotic cells with the most probability, while located in cytoplasmic mitochondria with a slight possibility. A nuclear export signal was found in ＂Arnt2 amino acids sequence, it was identified to be the leucine of No. 143 that located in N -terminal of Arnt2 amino acids sequence. Finally, the result of LSM analysis shows nuclear localization of Arnt2 in rat CGNs. CONCLUSION： Arnt2 is located in nuclei of normal rat CGNs, it suggests that Arnt2 has the tendency to translocate into mitochondria after induced by some of inducible factors, for both the possibility of mitochondria localization and NES exist in Arnt2 amino acids sequence.