兴奋性氨基酸抑制胎鼠神经干细胞的增殖

Influences of excitatory amino acids on the proliferation of neural stem cells from embryonic rat

目的:研究兴奋性氨基酸对体外培养胎鼠神经干细胞(NSCs)增殖的影响。方法:体外分离、培养与鉴定SD胚胎大鼠脑室下带(SVZ)神经干细胞,利用MTT比色法测定兴奋性氨基酸N-甲基-D-天冬氨酸(NMDA)和谷氨酸(Glu)对细胞增殖的作用。结果:成功分离出具有胚胎源性的神经干细胞,NMDA和Glu均能导致神经干细胞的增殖活性下降。结论:培养的SVZ区域细胞具有自我更新和多分化潜能,是中枢神经系统干细胞,兴奋性氨基酸能有效地抑制神经干细胞的增殖。

AIM： To study the influence of excitatory amino acids on the proliferation of neural stem cells from the embryonic rat. METHODS ： The neural stem cells from subventricular zone （SVZ） of the embryonic SD rat were isolated, cultured and identified in vitro. The effects of two excitatory amino acids, N - methyl - D - aspartate （NMDA） and glutamic acid （Glu）, on cell proliferation were detected by MTt colorimetric assay. RESULTS： The cultured cells showed embryo - derived characteristic were neural Stem cells. NMDA and Glu significantly decreased the proliferation rate in all these cells. CONCLUSION： The subventriculcr zone cells have the selfrenew and multipotent differentiation potential, belong to neural stem cells of central nervous system. Excitatory amino acids, NMDA and Glu, efficiently inhibit the cell proliferation.