大鼠喉移植改进模型的研究

Research of modified rat laryngeal transplantation model

目的探讨大鼠喉移植改进模型的可行性。方法近交系F344大白鼠80只,分为2组,对照组采用Strome喉移植模型,实验组保留咽升动脉,供体大鼠双侧颈总动脉分别与受体大鼠颈总动脉和颈前静脉行端端吻合,比较2组术后动静脉通畅率及移植喉存活率。结果对照组的动、静脉通畅率和移植喉存活率分别为30%、15%、30%,实验组为75%、65%、80%,两组比较差异有显著性(P<0.05)。结论改进的大鼠喉移植模型提高了受体鼠和移植喉的存活率,能更好地进行喉移植抗免疫排斥研究。

Objective To study modified rat laryngeal transplantation model. Methods Eighty isogeneic histocompatible F344 rats were randomized into control and experimental groups. Strome model of laryngeal transplantation was established in the the control group, and in the experimental group, the ascending pharyngeal artery was preserved and the base of the tongue, larynx and pharyngolarynx were harvested as a complex allograft followed by end-to-end anastomosis of the both aUograft common carotid arteries with the recipient common carotid artery and the anterior jugular vein, respectively. The arterial and nenous patency rate and allograft viability rate were compared between the two groups. Results The artery and vein patency rates and graft survival rate were 30%, 15%, and 30% in the control group, and 75%, 65%, and 80% in the experimental group, respectively, showing significant difference between the two groups （P〈0.05）. Conclusion In modified rat laryngeal transplantation model, the allograft viability rate and vessel patency rate are improved, which provides a good model for immunological study of larynx transplantation.