尖锐湿疣患者HLA-DM基因多态性研究

HLA-DM gene polymorphism in Cantonese with condyloma acuminata

目的探讨尖锐湿疣(CA)患者的HLA-DM基因的多态性,寻找可能的易感基因。方法运用PCR-RFLP方法确定98例CA患者和93例正常人群中HLA-DM的基因型,分析DM与CA的关联性。结果检测到7种DMA与DMB等位基因,HLADMA*0101和DMB*0101等位基因在汉族CA患者中的频率显著性增高(P<0.05和P<0.01),而DMA*0102等位基因在CA患者中的频率显著性降低(P<0.01)。DMA和DMB基因型在CA与正常对照之间无显著性差别。结论HLADMA*0101和DMB*0101等位基因可能是汉族人CA的易感基因或与易感基因连锁。

Objective To study the polymorphism of HLA-DM gene in Cantonese patients with condyloma acuminata （CA） and determine the susceptible genetic factors of CA. Methods DMA and DMB typing was performed in 98 Cantonese patients with CA and 93 healthy controls using restriction fragment length polymorphism method. Results The gene frequencies of DMA＊0101 and DMB＊0101 were significantly higher in the patients than in the controls （P〈0.05 and P〈0.01, respectively）, and gene frequency of DMA＊0102 was lower in patients than in the controls （P〈0.01）. Genotype frequencies of HLA-DM showed no significant difference between CA patients and the controls （P〉0.05）. Conclusion DMA＊0101 and DMB＊0101 alleles may be the susceptibility genes or closely linked to the susceptibility gene in Cantonese patients with CA.