习惯性流产的病毒病因探讨

Investigation of viral pathogens contributing to habitual abortion

目的用多种技术方法研究探讨柯萨奇B组病毒(CBV)与习惯性流产的病毒病因。方法采用ELISA、RT-PCR和病毒分离对86例习惯性流产和40例人工流产妇女进行了CBV-IgM抗体、病毒RNA和病毒粒子检测。结果检测结果表明习惯性流产和人工流产两组妇女柯萨奇B组病毒IgM阳性率分别为87.2%和35.0%,血淋巴细胞CBVRNA阳性率则为53.5%和17.5%;而胎盘组织中病毒RNA阳性率各为59.3%和17.5%,胎盘组织中病毒分离阳性率在两组妇女各为41.9%和6.9%。其各项指标经!2检验,两组均有显著性差异(P<0.01)。结论柯萨奇B组病毒可能是习惯性流产的发生因素之一。

Objective To investigate the association of Coxsackie B virus （CBV） with habitual abortion. Methods CBV IgM antibody, viral RNA and virions were detected in 86 women with habitual abortion and 40 with induced abortion by means of enzyme-linked immunosorbent assay （ELISA）, RT-PCR and virus isolation, respectively. Results The positivity rate of CBV IgM were 87.2% and 35% in the two groups, respectively, and the detection rate of the viral RNA was 53.5% and 17.5% in blood lymphocytes, and 59.3% and 17.5% in the placentas. The virions were found in the placentas in 41.9% and 6.9% of the women, respectively. The positiivty rates of CBV IgM, viral DNA and virions showed significant difference between the two groups （P〈0.01）. Conclusion CBV might be one of the causes responsible for habitual abortion.