肝炎颗粒对肝损伤的保护及免疫促进作用研究

Protective Effects of Ganyan Granule on Liver Injury and Its Immunomodulating Effects

目的:探讨肝炎颗粒对肝损伤的保护作用及免疫促进作用。方法:设对照组和药物组,采用四氯化碳(CCl4)和硫代乙酰胺(TAA)灌胃造成实验动物肝损伤,检测各组动物血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)水平,观察肝组织病理学变化;检验各组动物巨噬细胞的吞噬作用和血清溶血素水平。结果:肝炎颗粒能降低肝损伤所致实验动物血清ALT、AST、ALP升高的水平,其中高剂量组有显著性差异(P<0.05),肝组织损伤明显缓解;肝炎颗粒还能提高小鼠碳粒廓清指数和血清溶血素水平(P<0.05,P<0.01)。结论:肝炎颗粒对肝损伤有较好的保护作用及免疫促进作用。

Objective： To study the protective effects of Ganyan granule, a traditional Chinese medicine, on liver injury and its immunomodulating effects. Methods： Acute liver injury mice models were built up by Carbontetrachloride （CCl4） and thioacetamide （TAA）, and the mice were randomly assigned to the control and Ganyan granule treated groups. The ALT, AST and ALP levels in blood serum were detected by UV-VIS spectroscopy and the pathological observation of liver tissue was analyzed. The carbon expurgatory index, phagocytic index and the serolysin level were determinated. Results： Ganyan Granule could decrease the ALT, AST and ALP levels in rats serum with liver injury in high-dose group （all P〈0.05）. The pathological injury of liver tissue was alleviated markedly. Ganyan granule increased the carbon expurgatory index and the serolysin level （P〈0.05 and P〈0.01, respectively）. Conclusion： Ganyan Granule has more protective effects on liver injury and good enough immunomodulating effects.