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5-LOXmRNA和COX-2mRNA在胰腺癌中的表达及其临床意义 被引量：1

The expressions of 5-LOX mRNA and COX-2 mRNA in pancreatic cancer and their clinical significance
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摘要 目的研究5脂氧合酶(5-LOX)在胰腺癌中的表达,探讨其与环氧合酶2(COX-2)的关系。方法用RT-PCR检测5-LOXmRNA和COX-2mRNA基因在3 5例胰腺癌新鲜组织中的表达。结果5-LOXmRNA和COX-2mRNA在胰腺癌组织中的表达率分别为7 4.3%及8 0.0%。两者无协同作用(P>0.0 5)。5-LOXmRNA与胰腺癌的TNM分期有关,III,IV期5-LOXmRNA表达明显高于I,II期(P<0.0 5)。结论5-LOXmRNA和COX-2mRNA在胰腺癌中表达增高,与临床分期有关。两基因的表达无相关性。 Objective To investigate the expression of 5- LOX in pancreatic cancer tissue and the relationship between expression of 5-LOX and COX-2. Methods The expression of 5-LOXmRNA and COX-2mRNA in 35 fresh pancreatic cancer tissue samples were detected by semi-quantitive reverse transcriptase-polymerase chain reaction method. Results Expression of 5-LOX mRNA and COX-2mRNA in pancreatic cancer tissue was 74. 3% , 80% , respectively. Expressions of 5-LOX mRNA and COX-2 mRNA were not coordinated （ P 〉0.05 ）. There was correlation between the expression of 5-LOX and TNM stages. The expression of 5- LOX in stages Ⅲ- Ⅳ of pancreatic cancer was markedly higher than that in stages Ⅰ --Ⅱ of pancreatic cancer（ P 〈 0. 05 ） . Conclusions Expression of 5-LOX and COX-2 in pancreatic cancer tissue was markedly elevated, and correlated with clinical stage. Expressions of 5-LOX and COX-2 in pancreatic cancer were not coordinated.
作者 吕丽红 张波 曾庆东 徐霞
机构地区 山东大学医学院手术学研究室 山东省肿瘤防治研究院肝胆外科 山东大学齐鲁医院普通外科 山东省建筑医院
出处 《中国普通外科杂志》 CAS CSCD 2006年第6期419-422,共4页 China Journal of General Surgery
关键词 胰腺肿瘤/诊断 RNA 信使/遗传学 5脂氧合酶 环氧合酶2 Pancreatic Neoplasms/diag RNA, Messenger/genet 5-LOX COX-2
分类号 R735.9 [医药卫生—肿瘤] Q58 [生物学—生物化学]
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参考文献5

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同被引文献14

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  • 9Merati K,Said SM,Andea A,et al.Expression of inflammatory modulator COX-2 in pancreatic ductal adenocarcinoma and its relationship to pathologic and clinical parameters[J].Am J Clin Oncol,2001,24(5):447-452.
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中国普通外科杂志
2006年 第6期

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