细胞角蛋白(CK)13在含牙囊肿衬里上皮的表达

Cytokeratin(CK) 13 protein expression in the epithelial linings of dentigerous cysts

目的研究纤毛柱状上皮向鳞状上皮化生时细胞角蛋白13的表达,探讨其表达在上皮化生方面的意义。方法取54侧含牙囊肿和6例鼻腭管囊肿。分别采用细胞角蛋白13单克隆抗体进行免疫组织化学染色、HE染色和RT-PCR方法研究细胞角蛋白13基因的表达。结果54例含牙囊肿中,46例为复层鳞状上皮细胞衬里。CKl3均呈阳性表达;8例混合性上皮衬里。其中的鳞状上皮细胞部分CKl3也呈阳性表达;纤毛柱状上皮部分和6例鼻腭管囊肿的纤毛柱状上皮细胞均呈阴性表达。RT-PCR结果显示细胞角蛋白13基因在复层鳞状上皮中表达最强。在混合性上皮中表达减弱,在纤毛柱状上皮中表达极弱。结论细胞角蛋白13基因(CKl3-mRNA)在纤毛柱状上皮、混和性上皮和复层鳞状上皮三种不同上皮村里中均有表达,但表达强度不同,呈由弱到强的变化。在鳞状上皮细胞中细胞角蛋白13呈强阳性表达;在纤毛柱状上皮细胞中细胞角蛋白13呈阴性;可是,当纤毛柱状上皮中出现鳞状上皮细胞时,可见细胞角蛋白13呈阳性表达,因而,细胞角蛋白13是纤毛柱状上皮细胞向鳞状上皮细胞化生过程中的标志性产物。但是有待进一步验证。

Objective To investigate expression of CK13 when a metaplastic change occurs from ciliated columnar cells to squamoos cells. Methods Fifty - four cases of dentigeroos cysts and 6 cases of nasal cysts were immunohistochemically stained against CK13 monoclonal antibody. The expression of CK13 - mRNA was examined by RT - PCR in 28 cases of cysts. Results All 54 cases of squamoos cells expressed CK13. Eight cases of dilated columnar cell epithelia in upper jaws expressed CK13 in some areas of the columnar cell epithelium, though very few. As shown by RT - PCR CK13 - mRNA was expressed most strongly in squamoos cell epithelium. The CK13 - mRNA expression levels in the linings which consisted of both columnar cells and squamous cells were higher than those in linings of columnar cells only. Conclasion CK13 - mRNA expresses in three different epithelial linings： ciliated columnar epithelium, mingled epithelium （containing both ciliated columnar cells and squamous cells） and striated squamous epithelium, but the expression was at different level from weak to strong. CK 13 was positive in metaplastic squamous cells, but negative in dilated columnar cells. However, CK13 was expressed when squamous cell epithelium was found in ciliated columnar epithelium, CK 13 was expressed. Therefore, CK13 can be regarded as the marker of a metaplastic change from ciliated columnar cells to squamous cells. Yet, as an initial marker, it needs further examination.