摘要
背景与目的:观察给予溴氰菊酯(deltamethrin,DM)后大鼠大脑皮层、海马及下丘脑IL-1β的变化,初步探讨DM对神经免疫功能的影响。材料与方法:雄性SD大鼠共65只,其中15只用于RT-PCR,25只用于免疫组化,另25只用于ATPase活力测定,均随机分为1个对照组和4个DM不同作用时间的实验组。实验组中大鼠一次性腹腔注射溴氰菊酯12.5mg/kg,给药后1、6、24和48h分别检测上述3个脑区中IL-1βmRNA表达和蛋白含量的变化,同时以Na+-K+-ATPase为神经毒性指标,测定上述各时间点3个脑区Na+-K+-ATPase活力,探讨DM不同作用时间与IL-1β之间的关系。结果:皮层IL-1β含量在给药后1~6h升高,而IL-1βmRNA表达的增加在24h^48h与对照组相比差异有统计学意义(P<0.01),但未观察到ATPase活力的变化;给药后1h海马IL-1β含量升高,随之在6h时ATPase活力降低,在24h时IL-1βmRNA出现短暂升高和ATPase活力的恢复,48hATPase活力再度降低;下丘脑IL-1βmRNA的表达在给药后1h开始升高,6h时达峰值,IL-1β蛋白含量24h后才升高,ATPase活力仅在1h时一过性降低。结论:在DM对大鼠中枢神经系统不同脑区的毒作用过程中IL-1β可能发挥不同的作用。
BACKGROUND ＆ AIM： To observe the effects of deltamethrin（DM） on the neuroimmunological functions of rats by the changes of IL-1β in the cortex, hippocampus and hypothalamus. MATERIAL AND METHODS： 65 male adult SD rats were separated into 3 groups, one group included 15 rats for RT-PCR, the second group included 25 rats for immunohistochemistry, and the third included 25 rats for detecting the activities of ATPase. Each group was separated randomly into 5 sub-groups including 1 control and 4 DM treated. The DM treated rats received intraperitonea injection of 12.5 mg/kg DM. At 1, 6, 24 and 48 h after injection, we used RT-PCR and immunohistochemistry to characterize the changes of IL-1β mRNA expression and the protein content in different encephalic regions. Then we detected the activities of Na^＋ -K^＋ -ATPase as biomarker to explore the relationship between IL-1β and neural toxicity of DM.RESULTS： From 1 h to 6 h after DM administration, the IL-1β protein content in cortex increased while the gene expression of IL-1β rose after 24 h to 48 h, without neural toxicity in cortex. In the hippocampus, after IL-1β protein increased at 1 h with transient increase of IL-1β mRNA. The activity of Na^＋ -K^ -ATPase decreased at 6 h, and recovered at 24 h, but decreased again at 48 h. The IL-1β mRNA of hypothalamus increased rapidly in 1 h, reaching maximum at 6 h, whilst the IL-1β protein remained higher than that of the control group until 24 h. The activity of Na^＋ -K^＋ -ATPase only showed an apparent reduction at 1 h after injection. CONCLUSION： IL-1β may play different roles in above-mentioned cerebral regions in mediating the neural toxicity of DM in rats.
出处
《癌变．畸变．突变》
CAS
CSCD
2006年第4期261-264,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金项目(No.30371225)
