葡多酚对肝癌细胞增殖活性与Ca^(2+)浓度的影响

Effects of Grape Procyanidins on the Proliferative Activity and the Conc entration of Intracellular Calcium of Hepatoma Cells

背景与目的:研究葡多酚(Grapeprocyanidins,GPC)对肝癌细胞增殖活性与细胞内Ca2+浓度的影响。材料与方法:将人肝癌细胞与10、50和100mg/L3组不同浓度的GPC共同培养,用MTT(Methylthiazolyltetrazolium,MTT)法测定肝癌细胞的增殖活性,用Fura_2荧光法测定细胞内Ca2+浓度。结果:肝癌对照组细胞增殖活性和胞内Ca2+浓度分别为(0.445±0.024)nmol/L和(130.53±17.48)nmol/L,3个GPC剂量组的细胞增殖活性随GPC剂量升高显著性降低,而Ca2+浓度则显著性升高(t>2.78,P<0.05),其中50和100mg/L组均达Ca2+超载状态。结论:GPC可降低人肝癌细胞的增殖活性,引发胞内Ca2+浓度异常升高。

BACKGROUND ＆ AIM： To investigate the effects of grape procyanidins on the proliferative activity and the concentration of intracellular calcium of hepatoma cells. MATERIAL AND METHODS： Human hepatoma cells were cultured with differrent concentrations of GPC. The proliferative activity and concentration of intracellular calcium of the hepatoma cells were measured by methyl thiazolyl tetrazolium（MTT） assay and micmfluorimetric techniques based on calcium indicator Fura-2/AM.RESYKTS： The proliferation level and concentration of intracellular calcium of the control group were （0.045 ±0.024） nmol/L and （130.53 ± 17.48） nmol/L, respectively. The cell proliferation activitives of 3 GPC groups were lower, but the concentration of intracellular calcium was higher with increasing amounts of GPC（t 〉 2.78, P〈0.05） The concentration of intracellular calcium of both 50 mg/L and 100 mg/L GPC group were overloaded. CONCLUSION： GPC could inhibit the proliferative activity and abnormally elevate the concentration of intracellular calcium of hepatoma cells .