论著海生多糖肽对辐射诱发小鼠肝细胞POD和Caspase-3异常表达的影响

The Effect of HPP on Abnormal Expression of Mice Hepatic Cells POD and Caspase-3

背景与目的:探讨海生多糖肽(Haishenpolysaccharidepeptide,HPP)制剂对辐射引发小鼠肝细胞凋亡和凋亡基因异常表达的影响。材料与方法:将饲以150mg/kg和100mg/kgHPP制剂的小鼠用60Coγ射线全身亚急性照射,体内灌注固定肝组织,采用原位末端标记和原位杂交技术检测肝细胞辣根过氧化氢酶(Peroxidase,POD)和半胱氨酸蛋白酶-3(Caspase-3)的阳性表达水平。结果:辐射损伤组POD和Caspase-3阳性表达率分别为(40.0±3.5)%和(26.0±1.2)%,高剂量海生多糖肽组分别为(23.9±3.8%)和(6.0±1.5)%,差异有统计学意义(P<0.01)结论:海生多糖肽可抑制辐射引发的小鼠肝细胞POD和Caspase-3异常表达,抑制肝细胞损伤和凋亡。

BACKGROUND ＆ AIM： To study the effect of （Haishen polysaccharide peptide, HPP） on the apoptosis and apoptosis gene of mice hepatic cells induced by 60Co-γ irradiation . MATERIAL AND METHODS： Mice were fed 150 mg/kg and 100 mg/kg HPP for four weeks. Mice were then irradiated with 60Co-γ to induce hepatic cells apoptosis once a day five days a week, Four weeks later, in situ cell death detection kit and in situ hybridization were applied to measure qualitative changes at expression level of POD and Caspase-3 positive cells rate to analyze the apoptosis of mice hepatic cells. RESULTS： The rates of POD and Caspase-3 expression in the radiation-injury group were（40.0± 3.5）% and（26.0±1.2）%, respectively, and the rates of the high dose group were（23.9± 3.8）% and （6.0±1.5）%, respectively, the difference between the radiation-injury group and high dose group showed statistical significance （P〈0.01） .CONCLUSION： HPP had a significant inhibitory effect on the abnormal hepatic expression of POD and Caspase-3 induced by radioactive damage.