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WI-L2家族人类淋巴母细胞TK基因突变研究 被引量：1

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摘要 WI-L2家族主要包括有TK6、WTK1、TK6-E6三种细胞株,其TK(Thymidinekinase,TK)基因均为杂合型(TK+/-),但P53状态各不相同。三种细胞的TK基因突变实验广泛用于P53功能研究。考虑到三种细胞培养简单而且是人类来源,目前该试验也逐步应用于多种物质遗传毒性检测。三种细胞的TK试验的差异丰富了对毒作用表现形式的认识,也为探讨遗传毒作用机制提供了崭新的视角,是遗传毒性研究中很好的生物材料。因此对三种细胞的TK实验差别及其相关研究做了简要综述。
作者 张勇 张立实
机构地区 重庆医科大学公共卫生学院营养与食品卫生教研室 四川大学华西公共卫生学院营养与食品卫生教研室
出处 《癌变．畸变．突变》 CAS CSCD 2006年第4期331-333,共3页 Carcinogenesis,Teratogenesis & Mutagenesis
关键词 类淋巴母细胞 TK基因 突变实验
分类号 Q335 [生物学—遗传学]
  • 相关文献

参考文献34

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二级参考文献2

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共引文献8

同被引文献10

  • 1张勇,张立实.两种人类淋巴母细胞TK、HPRT基因突变实验比较研究[J].癌变．畸变．突变,2007,19(1):36-39. 被引量：4
  • 2Masamitsu H,Toshio S.The mouse lymphoma assay (MLA) using the microwell method[M].New York:Marcel Dekker Inc,2001.141-161.
  • 3Livak K J,Schmittgen S T.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method.[J].Methods,2001,25(4):402-408.
  • 4Pongsaensook P,Smith L E,Grosovsky A J,et al.Isolation of an APRT heterozygote from TK6 human lymphoblasts[J].Mutat Res,1997,377(1):27-36.
  • 5Morimoto S,Honma M,Yatagai F.Effect of p53 codon 237 mutation on X-ray induced HPRT mutations[J].Environ.Mutagen Res,2002,24 (1):17-21.
  • 6Morimoto S,Honma M,Yatagai F.Sensitive detection of LOH events in a human cell line after C-ion beam expose[J].Radiat.Res,2002,43:163-167.
  • 7Morimoto S,Kato T,Honma M,et al.Detection of genetic alternations induced by low-dose X rays[J].Radiat Res,2002,157(5):533-558.
  • 8Umebayashi Y,Honma M,Suzuki M,et al.Mutation induction in cultured human cells after low-dose and low-dose-rate gamma-ray irradiation detection by LOH analysis[J].Radiat Res,2007,48(1):7-11.
  • 9Dietrich N,Bracken AP,Trinh E,et al.Bypass of senescence by the polycomb group protein CBX8 through direct binding to the INK4A-ARF locus[J].EMBO J,2007,26(6):1637-1648.
  • 10Merrill J C,Melhuish T A,Kagey M H,et al.A Role for Non-Cova-lent SUMO Interaction Motifs in Pc2 CBX4 E3 Activity[J].PLOS One,2010,5(1):879.

引证文献1

癌变．畸变．突变
2006年 第4期

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