血管内皮生长因子在寻常型银屑病患者中医辨证分型皮损和血清中的表达

Expression of VEGF in Skin Lesions and Serum Level in Patients of Psoriasis Vulgaris with TCM Differentiation-types

目的探讨血管内皮生长因子(VEGF)在寻常型银屑病患者中医辨证分型(血热型、血燥型和血瘀型)皮损和血清中的表达。方法采用免疫组化法和酶联免疫吸附法(ELISA法)对60例寻常型银屑病患者(中医辨证分型)皮损处及血清中VEGF的水平进行了检测。结果皮损处VEGF水平为血热型组>血燥型组及血瘀型组;血清中VEGF的水平为血热型组>血燥型组>血瘀型组。结论对寻常型银屑病患者皮损处及血清中VEGF水平的检测有可能用于血热型银屑病与非血热型银屑病的中医辨证分型。

Objective To explore the relation between the changes of VEGF in skin lesions and serum level in patients of psoriasis vulgaris with TCM differentiation-types （ Blood Heat, Blood Dry and Blood Stasis）. Methods Sixty cases of psoriatic lesions and sera with TCM differentiation-types were studied. Expression of VEGF was detected by immunohistochemistry and enzyme-linked immunosorbent assay （ELISA） in psoriatic lesions and sera in patients of psoriasis vulgaris with TCM differentiationtypes. Results Comparing the datas of normal control,the expression of VEGF in psoriatic lesions was significantly increasea （P 〈 0.001 or P 〈 0.05 ）, particularly in ＂Blood Heat＂ type of psoriatic lesions as compared to those in other two types （ P 〈 0.001 ）. The serum level of VEGF in patients with ＂Blood Heat＂type was also significantly increased as compared to those in normal control and other two types （ Blood Dry and Blood Stasis）. Conclusion The detection of VEGF both in skin lesions and serum level may be available to distinguish ＂Blood Heat＂ type from ＂Non-Blood Heat＂ type （Blood Dry stasis and Blood stasis） in patients with psoriasis vulgaris with TCM differetiation-types