川芎嗪改善高渗枸橼酸盐嘌呤溶液保存犬肾的效果

Effect of ligustrazine on canine kidney preservation

目的探讨一定浓度的川芎嗪对低温保存的犬肾的影响。方法分别以2～4℃加入川芎嗪（终浓度为4mg／L）的新型高渗枸橼酸盐嘌呤溶液（HC-A Ⅱ液，实验组）、HC-A Ⅱ液（HC-AⅡ液组）及UW液（UW液组）灌注肾脏，然后进行组织病理学观察及细胞凋亡测定。将按上述方法保存48、72h的肾脏进行自体移植，术后观察血肌酐浓度、恢复正常的时间以及受者的存活情况。结果体外保存实验中，在保存时间不超过48h时，三个组的组织形态基本相似，但保存72h后，实验组和UW液组肾组织的病理改变明显轻于HC-A Ⅱ液组，细胞凋亡指数也明显低于HC-A Ⅱ液组（P〈0．05），实验组和UW液组的上述指标相比较，差异无统计学意义（P〉0．05）。经保存的肾脏自体移植后，单纯HC-A Ⅱ液保存72h者有2只犬（2／5）因肾功能衰竭死亡；不论是保存48h还是72h，采用含川芎嗪的HC-AⅡ 液和UW液保存者移植后血肌酐水平明显低于单纯HC-A Ⅱ液保存者（P〈0．05），其肾功能恢复正常的时间也较单纯HC-AⅡ液保存者缩短（P〈0．05）。结论HCA-Ⅱ液中加入一定浓度的川芎嗪能改善肾脏低温灌注保存的效果。

Objective To investigate the effect of ligustrazine with a certain concentration added to HCA-Ⅱ solution on kidney preservation. Methods In the experimental group, ligustrazine （4 mg/ L） plus HCA-Ⅱ solution were administered, and in control groups HCA-Ⅱ solution and UW solution were given. The isolated canine kidneys were flushed and stored at 0-4 ℃for 24, 48 and 72 h. The renal cortex was taken for morphological and ultrastructure examinations. Indexes of renal cell apoptosis were calculated. Serum creatinine （ser） was measured before and after renal transplantation. Results Light microscopic and electron microscopic studies revealed that histomorphological changes of kidneys in experimental group were much milder than in HCA-Ⅱ solution group （P〈0. 05）. The indexes of renal cell apoptosis in experimental group were lower than in HCA-Ⅱ solution group （P〈0. 05）. The peak value of Scr after transplantation was higher and the time for Scr to normal value was longer in experimental group than in HCA-Ⅱsolution group （P〈0. 05）. There was no difference between experimental group and UW solution group （P〉 0. 05 ）. Conclusion The ligustrazine with this concentration in HCA-Ⅱ solution has protective effects on hypothermia perfusion and preservation of isolated kidney.