口腔鳞癌中IL-8的表达及其与微血管生成的关系

Relation of Interleukin-8 Expression with Angiogenesis in Human Oral Souamous Cell Carcinoma

目的探讨白细胞介素-8(IL-8)的表达与口腔鳞癌(OSCC)血管形成和生物学行为的关系及意义。方法应用免疫组织化学技术检测55例OSCC手术切除标本和10例正常口腔粘膜标本中IL-8的表达及肿瘤内的微血管密度(iMVD)。结果口腔鳞癌标本中32例(58.18%)IL-8呈阳性表达,口腔鳞癌微血管密度为38.58±12.43;IL-8评分为高级的口腔鳞癌组织iMVD高于IL-8评分为阴性和低级的口腔鳞癌组织。iMVD与IL-8呈正相关(rs=0.699,P<0.01)。结论IL-8表达与肿瘤内iMVD明显相关,同时与口腔鳞癌的病理分级、临床分期及淋巴结转移关系密切。IL-8很可能以促血管生成因子的角色在口腔鳞癌的发生、发展中起重要作用。

Objective To explore the expression of interleukin-8 （IL-8） and it＇s relation with angiogenesis and tumor biological behavior in human oral squamous cell carcinoma（OSCC）. Methods The expression of IL-8 and the intratumoral microvessel density （iMVD） in 55 cases of human oral squamous cell carcinoma and 10 case of normal lingual mucosa were examined by means of immunohistochemical method. Results The positive staining for IL-8 was obtained in 32 cases of the OSCC（58. 18%）,and iMVD was 38. 58 ± 12. 43/× 400 in OSCC. The iMVD in high-grade IL-8tumors were significantly higher than in IL- 8-negative and low-grade IL-8 tumors, and iMVD was positively correlated with IL-8 expression（ rs = 0. 699, P〈0. 01）. Conclusion These results suggested that IL-8 expression is significantly associated with iMVD, tumor grade, clinical staging and lymph node metastasis of human oral squamous cell carcinoma. IL-8 may play an important role in the occurrence and development of OSCC.