五氯硝基苯对小鼠脑AChE的影响及多器官氧化损伤研究

STUDY ON THE ACTIVITY OF ACETYLCHOLINESTERASE AND OXIDATION DAMAGE ON DIFFERENT ORGANS OF MICE INDUCED BY PENTACHLORONITROBENZENE

测定五氯硝基苯(PCNB)对小鼠脑乙酰胆碱酯酶(AChE)的影响及多器官的氧化损伤作用.对昆明种小鼠采用灌胃法进行200,400和600 mg.kg-1三个剂量染毒,每日一次,分别于染毒4,8,12和16d测定AChE活性、过氧化氢酶(CAT)活性和脂质过氧化(LPO)产物丙二醛(MDA)的含量.结果表明:(1)小鼠脑AChE活性受到抑制,并表现为明显的时间和剂量依赖性;(2)肝、肾CAT活性变化显著,脂质过氧化作用明显;(3)高剂量毒物在染毒16d时对脾脏脂质过氧化作用明显增强;(4)脑和血清CAT活性随染毒时间延长先升高后降低,脂质过氧化作用不明显.PCNB对小鼠可能具有神经毒性,对肝、肾脏的氧化损伤作用较强.

The effects of pentachloronitrobenzene （ PCNB ） on the activity of acetylcholinesterase （ AChE ） in brain and oxidation damage in various organs （brain, liver, kidney, spleen and serum） of mice were studied. The mice were poisoned at different concentrations （200, 400, 600 mg · kg^-1 ）, once a day . The activities of AChE and catalase （CAT） and the levels of lipid peroxidation （LPO） of the organs were measured at 4, 8, 12 and 16 days. The results showed that （ 1 ） the activities of AChE were inhibited and showed an evident dependence on dose and time ; （2） the activities of CAT in liver and kidney changed evidently and the levels of LPO were obvious; （3） after treated 16 days, the levels of LPO of spleen increased at the concentration of 600 mg ·kg^-1; （4） in brain and serum, the activities of catalase （CAT） increased first and then decreased following the PCNB concentrations varied from low to high. But the levels of LPO were not serious. PCNB could have nerve toxicity and oxidation damage on liver and kidney.