原发性胆囊癌P53基因突变产物的表达及其意义

Expression of P53 mutant protein and clinical significance in gallbladder caroinoma

作者应用ＡＢＣ免疫组化法研究４０例胆囊癌和５例正常胆囊粘膜标本中突变型Ｐ５３蛋白的表达及其意义。结果显示胆囊癌中突变型Ｐ５３蛋白表达阳性率为５０％，正常胆囊粘膜全部为阴性。突变型Ｐ５３蛋白的表达阳性率在不同的病理分级、淋巴结是否转移及预后患者间差异显著（Ｐ＜０．０１）。在Ｎｅｖｉｎ分期间未测出显著相关性（Ｐ＞０．０５）。结果提示突变型Ｐ５３基因参与了胆囊肿瘤的恶性转化过程，其表达阳性率高的胆囊癌预后不良，是反应胆囊癌生物学行为和预后的重要指标。

The expressions and sinificance of mutant P53 protein were studied by immunohi stochemical method in 40 casses with gallbladder carcinoma and 5 casese with normal chelecyst.The protein positive rate of was P53 mutant protein 50% in cancer patient,and all the negative in normal gallbladder.The positive rate was significantly different a cording to pathological grade,with or without node metastasis and porgnosis)P<0.01).There were no significant correlations between the Nevin classifications (P>0.05).Thses results suggest the mutant P53 gene participates in transformation of carcinoma, and gallbladder carcinona with highly positive rate might have poor prognosis and P53 might be an important marker reflecting the biological behavior and prognostic value.