esp1基因过表达可诱导肿瘤细胞凋亡

UP-REGULATED esp1 GENE EXPRESSION COULD INDUCE APOPTOSIS IN A549 CELL

目的研究esp1基因转染进入A549细胞后对细胞凋亡的影响。方法将含esp1基因的IRE-EGFP质粒经FUGENE 6转染试剂转染到肺腺癌细胞A549,用倒置显微镜及流式细胞仪检测转染后细胞染色体数以及转染后细胞凋亡及增殖的情况。结果esp1基因导入A549细胞后,染色体数目减少,无血清培养后细胞凋亡增加,增殖减少。结论esp1基因在A549细胞中不但可以显著地改善细胞的非整倍性而且可以有效地抑制细胞恶性增殖、促进细胞凋亡,从而为肺癌的治疗提供了一个新的靶标。

Objective To study the influence of espl gene transfection on apoptosis of A549 cell line in vitro. Methods IRE-EGFP plasmid containing espl gene was transfected into adenocarcinoma of lung cell line A549 via FUGENE 6. Chromosomes in transfected cells were analyzed under invert microscope and cell apoptosis and proliferation were studied with Flow cytometry. Results Transfection of espl into A549 cells resulted in decreased number of chromosomes. After being cultured without serum, apoptosis increased while cell proliferation was suppressed. Conclusion The induction of espl gene into A549 cell line can effectively depress aneuploid, inhibit proliferation and promote apoptosis of tumor cells, indicating espl may be a new potential target for the treatment of lung cancer.