针刺对新生鼠缺血缺氧性脑损伤皮质Nestin表达的影响

The influence of acupuncture on Nestin expression in the cortex of hypoxia-ischemic neonatal rats

目的观察针刺对新生鼠缺血缺氧性损伤后皮质神经干细胞的影响。方法新生7d龄SD大鼠72只,随机分为正常组(24只)、新生鼠缺血缺氧性脑病(HIE)模型组(24只)和HIE+针刺组(24只),3组均于建模后第3,7,14,28天处死取脑,行免疫组化染色,巢蛋白(Nestin)标记神经干细胞。结果正常SD大鼠生后早期的皮质存在一定数量的Nestin阳性细胞,随年龄增加而减少；缺血缺氧后,新生SD大鼠皮质损伤区及其周围、对侧镜区出现Nestin阳性细胞增多,并以病灶边缘更加突出,伤后3d达高峰,HIE+针刺组阳性细胞明显增多(P＜0．01),并使高峰时间延长,高峰期推迟,至伤后28d各组无差异。结论针刺可促使新生SD大鼠缺血缺氧性损伤后皮质神经干细胞增多,高峰期延长。

Objective To investigate the influence of acupuncture on Nestin expression in the cerebral cortex of hypoxia-ischemic neonatal rats. Methods A total of 72 Sprague-Dawley（SD） rats aged 7 days were randomly divided into three groups： A normal group（ n = 24）,the hypoxic-ischemic encephalopathy（HIE） group （n = 24）, and an acupuncture ＋ HIE group（ n = 24）. The rats were sacrificed at time points including 3d, 7d, 14d and 28d after insult, their brains were removed and sections were made. Any nerve stem cells （NSCs） in the cerebral cortex were detected using immunohistochemical staining of Nestin. Results Nestin-positive cells were found in the cerebral cortex in the normal neonatal SD rats early after birth. After HI insult, the Nestin-positive cells had increased in the injured areas of the cortex and in eontralateral mirror areas, especially around the injured areas. Peak Nestin expression was 3 days after insult. In the acupuncture ＋ HIE group, Nestin positive cells were significantly increased when compared with that in the HIE group, the peak was postponed, and the peak was longer than in the HIE group. The Nestin positive cells decreased, were seldom seen, and were not different among three groups 28 days after the HI insult. Conclusions Endogenous neural stem cells in the cerebral cortex were increased by acupuncture in hypoxia-ischemic in neonatal rats, and acupuncture made the peak of Nestin expression longer.