高强度超声对兔肝肿瘤微血管损伤作用的病理观察

The effect of high intensity ultrasound on micro-vessels in a tumor in a rabbit liver

目的观察高强度超声(HIU)对肿瘤细胞和增生血管的破坏作用。方法采用HE染色、内皮细胞生物素标记的荆豆凝集素Ⅰ(UEAI)免疫组织化学染色和电镜检查,观察HIU对兔VX_2肝脏肿瘤营养血管破坏的情况。结果HE染色显示,HIU治疗后肿瘤营养血管和肿瘤组织发生凝固性坏死,靶组织UEAI染色为阴性,电镜观察肿瘤微血管结构完全破坏。结论HIU能破坏肿瘤营养血管,防止血管再生,从而有可能抑制肿瘤的再生和转移。

Objective To evaluate the damage caused by high intensity ultrasound （HIU） on tumor microvessels. Methods Rabbit models of the VX2 tumor were set up. The target hepatic carcinomas were treated with HIU, and the results were observed using hematoxylin-eosin （HE） staining, vascular endothelial cell biotinylated-ulex europaeus agglutinini I （UEAI） immunohistochemical staining, tumor micro-vessel counts, and electron microscopy. Results Histological examination through HE staining indicated that HIU induced carcinoma vaseularities with whole tumor tissue coagulative necrosis. The UEAI immunohistochemical staining of the target lesions treated by HIU was negative, and complete tumor micro-vessel ultrastructure damage was observed under the electron microscope. Conclusion HIU can damage tumor micro-vessels thoroughly, in such way that it may inhibit tumor growth and metastasis.