期刊文献+

硝化悬浮填料塔中氨氧化细菌群落稳定性特征分析 被引量:3

STABILITY ANALYSIS OF AOB COMMUNITY IN NITRIFYING SUSPENSION FILLER TOWER
下载PDF
导出
摘要 利用PCR-DGGE分析技术,对于化学生物絮凝处理工艺中悬浮填料塔的氨氧化细菌(AOB)群落进行了研究。主要考察了曝气强度和水力停留时间2个运行参数调整的情况下,系统AOB群落结构的变化。生物膜样品直接取自硝化填料塔,多维尺度(MDS)方法用于DGGE电泳图谱的分析,分析结果以二维图形向量的方式显示,结果表明,曝气强度和水力停留时间均对AOB群落有一定的影响,主要的AOB种类在运行过程中基本不变,系统对于运行条件具有良好的稳定性。 It was studied that ammonia-oxidizing bacteria(AOB) community in a nitrifying suspension tower for biochemical flocculation process,using PCR-DGGE technique.The change in systematic AOB community structure was examined under the regulation of aeration strength and hydraulic residence time(RHT).The biofilm samples were taken directly from a nitrifying filler tower.MDS method was used for the analysis of DGGE electrophoretogram,and the results were displayed by 2-D pattern.The results show that the aeration strength and RHT have a certain effect on AOB community.The main AOB strains remain unchanged in the process of running.
出处 《环境工程》 CAS CSCD 北大核心 2006年第3期10-12,共3页 Environmental Engineering
基金 上海市曙光计划课题(03SG20) 高等学校博士点基金课题(20050247016)
关键词 聚合酶链式反应 浓度梯度凝胶电泳 氨氧化菌(AOB) polyase chain reaction,concentration gradient of gel electrophoresis and AOB
  • 相关文献

参考文献5

二级参考文献20

  • 1许建华,万英,汤利华,王白杨.微污染原水的生物接触氧化预处理技术研究[J].同济大学学报(自然科学版),1995,23(4):376-381. 被引量:60
  • 2BEDARD C, KNOWLES R. Physiology, biology and specific inhibitors of CH4+, NH4+ and CO oxidation by methanotrophs and nitrifiers [ J ]. Microbiol Rev,1989, 53(1): 68-84.
  • 3KLOTZ M G, ALZERRECA J, NORTON J M, et al. A gene encoding a membrane protein exists upstream of the anoA/amoB genes in ammonia oxidizing bacteria: a third member of the ano operon [J].FEMS Microbiol Lett, 1997, 150(1): 65-73.
  • 4MCTAVISH H, FUCHS J A, HOOPER A B. Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea[J]. J Bacteriol, 1993,175(8): 2436-2444.
  • 5SINIGALLIANO C D,KUHU D N, JONES R D.Amplification of the amoA gene from diverse species of ammonium-oxidizing bacteria and from an indigenous bacterial population from seawater[J]. Appl Environ Microbiol, 1995, 61(7): 2702- 2706.
  • 6ROTTHAUWE J H, WITZEL K P, LIESACK W.The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations[J]. Appl Environ Microbiol, 1997, 63(12): 4704-4712.
  • 7PURKHOLD U, POMMERENING-Roser A, JURETSCHKO S, et al. Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys[J]. Appl Environ Microbiol, 2000, 66(12): 5368-5382.
  • 8TSAI Y L, OLSON B H. Rapid method for direct extraction of DNA from soil and sediments [J]. Appl Environ Microbiol, 1991, 57(4): 1070-1074.
  • 9TSAI Y L, OLSON B H. Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction[J]. Appl Environ Microbiol, 1992, 58(7): 2292-2295.
  • 10Liu Z, 1993. Study on the MLSS concentration increasing in aeration tank[J]. Water Supply and Sewage Disposal, 19(8) : 17-19.

共引文献36

同被引文献53

引证文献3

二级引证文献41

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部