胰炎合剂治疗大鼠急性坏死性胰腺炎细胞核因子-к B活性的研究

Study on the activation of NF-к B in rats with ANP treated by yiyanheji

目的:研究Wistar大鼠急性坏死性胰腺炎(ANP)应用胰炎合剂,N-乙酰半胱氨酸,生理盐水治疗对血清淀粉酶的变化,胰腺的形态学改变,胰腺细胞NF-кB活性的影响。方法:健康Wistar大鼠150只,制备成急性坏死性胰腺炎模型,术后分别用胰炎合剂,N-乙酰半胱氨酸,生理盐水进行治疗。不同时间取血液标本测定血淀粉酶,观察胰腺组织光镜及电镜变化,胰腺标本行免疫组化检测胰腺组织细胞中核因子-кB(NF-kappaB)的活性。结果:各组模型血清淀粉酶水平明显上升,但胰炎合剂组及N-乙酰半胱氨酸组治疗后血清淀粉酶下降快,生理盐水组下降慢(P<0.05或P<0.01);各组形态学变化与核因子-кB活性变化相平行。结论:在急性坏死性胰腺炎发生和发展中核因子-кB起关键作用。用核因子-кB抑制剂N-乙酰半胱氨酸治疗能降低核因子-кB的活性,同时也减轻了胰腺的炎症程度,胰炎合剂治疗急性坏死性胰腺炎的机理和降低核因子-кB活性有关。

Objective：In order to study the variety of blood AMY,morphological change of pancreas and activation of gland cell in rats with acute necrotic pancreatitis （ANP） treated by yiyanheji,N-acetylcysteine and physiological saline.Methods：The ANP models were astablished in 150 healthy wistar rats,and treated with yiyanheji,N-acetylcysteine or physiological saline respectively.The serum level of AMY was determined,the pancreatic tissue change was observed by optical microscope and electron microscope and the activation of NF- kappa B （NF-κ B）was detected by immunohistochemistry in different times.Results：Althougy the serum level of AMP ascended in all model groups,but it declined rapidly in the group treated with ylyanheji and N-acetylcysteine compared with that in the group treated with physiological saline （P〈0.05 or P〈0.01 ）.The morphological change of pancreas in all model groups was paralleled with the change of the activation of NF-κB.Conclusion：The activation of NF-κB plays a key role in the development of ANP.N-acetylcysteine not only can inhibit the activation of NF-κ B,but also abates the inflammatory extent of pancreas.The mechanism of yiyanheji for treating ANP correlates with the depressed activtion of NF-κB.