肾细胞癌PCNA表达与CT征象关系的探讨

Primary study on the correlation between CT cppearances and PCNA expression in renal cell carcinoma

目的:研究肾细胞癌相关基因PCNA与CT征象的关系。方法:用免疫组化染色(S-P法)检测40例经手术切除、病理证实且CT资料完整的肾细胞癌和10例正常肾组织的PCNA阳性表达率。结果:(1)PCNA阳性表达率与肿瘤直径,肿瘤强化,肾周脂肪浸润有关,P<0.05;而与瘤体内坏死、液化,淋巴结肿大,静脉侵犯,邻近器官侵犯或(和)远处转移无关,P>0.05;(2)肿瘤病理分级增加,PCNA阳性表达率升高,P<0.05。结论:RCC的CT表现与PCNA的表达存在内在关系,据此可术前评价其生物学行为与恶性程度。

Objective：To study the correlation between CT appearances and the expression of PCNA in renal cell carcinoma. Methods：All 40 paraffin embedded specimens of randomly selected RCC patients were examined and diagnosed by CT,nephrectomy and pathology.The incidence in expression of PCNA gene was determined by immunohistochemical techniques with specific mono clonal antibodies.Results：（1） The positive expression of PCNA was significantly correlated with tumor size, enhancement extent, perirenal fat infiltration on CT scan （P〈0.05）; However,there was no significant difference in PCNA expression if tumors had intratumor necrosis, liquefaction,lymph nodes metastases,invasion of renal vein or inferior vena cava,invasion of adjacent organs or distant metastasis （P〉0.05）; （2）As nuclear grade increased,RCCs were more likely to be positive PCNA expression.（P〈0.05）.Condusion：The detection of PCNA gene expression in RCC combined with CT features was useful in early and exactly diagnosing of RCC and more objectively evaluating the biological behavior, malignance and prognosis of RCC.