生后不同时间大鼠侧脑室脑室下区Musashi1的表达

The expression of Musashi1 in the subventricular zone of lateral ventricles of rat at different postnatal developing times

目的观察神经特异性RNA结合蛋白Musashi1（Msi1）在大鼠生后不同发育时间侧脑室脑室下区（SVZ）内的表达变化。方法健康雄性SD大鼠，分为7天、2周、3周、1月、2月、成年（6月龄）和老年（18月龄）7组，每组5只，共35只，多聚甲醛灌注固定，冰冻冠状切片，免疫组织化学（ABC法）染色，光镜观察摄像，细胞计数。结果各年龄组大鼠侧脑室周围的SVZ内可见Msi1免疫反应阳性细胞，胞体小圆形，无突起或1-2个短突起，分布疏密不均匀；随年龄增长，SVZ内Msi1阳性细胞逐渐减少，老年组大鼠仍然有一定数量的Msi1阳性细胞。结论大鼠侧脑室SVZ内存在有Msi1阳性细胞，并随年龄增长而明显减少，这些细胞可能是神经干细胞。

Objective To observe the expression of neural RNA - binding proteins Musashil （Msil） in the subventricnlar zone （SVZ） of lateral ventricles （LV） at different postnatal developing times. Methods Healthy male SD rats of different age groups were recruited （ 1 - , 2- and 3 - week groups, 1 - and 2 - month groups, 6 - month grown group, and 18 - month aged group; n = 5 each）. Immunohistochemistry was performed on the coronal sections of brain of the rats perfused with pamformaldehyde. Results The expression of Msil was observed in the SVZ of LV in all groups. Msil - positive cells were small and round in shape, showing no or 1 - 2 short processes on the surface. The Musashil - positive cells were unevenly distributed. The number of them was reduced gradually with age. Conclusion Many Musashil - positive cells are present in the SVZ of rat the whole life, with their number reducing gradually with age. They might play as neural stem cells.