大鼠持久性肝硬化动物模型的建立

Long-term observation of CCL_4-induced live cirrhosis in rat

目的探讨建立持久性肝硬化动物模型的方法。方法将200只3月龄雄性Wistar大鼠，随机分成空白对照组（A组），药物对照组（B组）和药物联合肝中叶切除组（C组），用四氯化碳联合肝中叶切除术建立肝硬化模型，分7批，每隔三个月处死一批动物，分别记录各组每只动物的脾脏重量、门静脉宽度、睾丸重量、肝纤维化或肝硬化程度。结果三组动物脾脏重量依次增加，门静脉宽度依序增宽。而睾丸重量变化无明显规律。至21月时，C组S3＋S4仍为92％～100％，而B组则为50％～75％（P〈0．01）。结论四氯化碳／乙醇联合肝中叶切除术建立的大鼠肝硬化模型持久稳定。

Objectives To establish a rat persistent liver cirrhosis model. Methods Fore stablishing a liver cirrhosis induced by CCl4 plus resection of liver median lobe in rat, 200 three-month-old male Wistar rats were randomly divided into normal （A）, CCl4 （B）and CCl4/resection of liver median lobe （C）group. The rats were batched and sacrificed every three months. The spleen weights, portal vein widths, testes weights, the status of hepatic fibrosis and liver cirrhosis were assessed. Results The spleen weights and portal vein widths were increased from A to C group, whereas there was no difference in testes weights among those three groups. The rate of maintained status of hepatic fibrosis and liver cirrhosis was significantly higher in the C group than in the B group （P〈0. 01）. The former was 92% and 100% and the latter 50 % and 75 %. Conclusions It is steady to establish a rat liver cirrhosis model induced by CCl4/resection of liver median lobe for a relatively long times.