摘要
以秋水仙素处理中籼３０３７幼苗，从其后代中获得同源三倍体１株。为选育成套初级三体，将中籼３０３７同源三倍体茎节幼芽置于试管中离体繁殖，繁殖后的同源三倍体种于田间，授以正常中籼３０３７的花粉。获得３０１３粒种子，成苗１０６３株。通过染色体数目检查，共获得３０２株三体植株。这些三体按其形态特征可分为１２类，对每类三体植株分别通过粗线期的染色体鉴定，获得了１２种初级三体，依据额外染色体长度递减的顺序分别定名为三体１、三体２、………三体１２。
For developing primary trisomics a midseason variety of indica rice Zhongxian 3037 was treated with 0.2% colchicine solution at seedling stage. A triploid plant was obtained from its offspring in 1991. The triploid was in vitro propagated on culture medium. After pollenation with the pollen of diploid plants of the same variety, 3013 seeds were harvested in 1992. From these seeds 1063 plants were obtained and chromosome number checking was conducted for each plant in meiosis. A total of 302 plants were confirmed to be trisomic plants among which 12 primary trisomics were successfully isolated and being designated as triplo-1, triplo-2……and triplo-12 according to the length of additional chromosomes. Each primary trisomic was maintained in test tube with tissue culture techniques.This project supported by National Natural Science Foundation of China
基金
国家自然科学基金
