原核细胞表达的肾综合征出血热病毒核蛋白免疫原性及免疫保护作用的研究

Study on Immunogeneci ty and Immunoprotection of Hantaan Virus Nucleocapsid Protein Expressed in E.coli

对原核细胞表达的肾综合征出血热病毒核蛋白的免疫原性及免疫保护作用进行了研究，结果发现表达的核蛋白具有很强的免疫原性，免疫小白鼠血清中抗肾综合征出血热病毒荧光抗体滴度达１：１２８００；用免疫小鼠脾细胞和血情做被动免疫保护实验，结果显示脾细胞对部分乳鼠肾综合征出血热病毒的致死感染有保护作用，保护率可达３８％，而血清不具有免疫保护作用；同时也未观察到核蛋白免疫血清具有促感染乳鼠早死作用。表明核蛋白能诱导细胞免疫保护但不诱导抗体介导的免疫保护，同时也不引起抗体介导的早死，这就为肾综合征出血热病毒核蛋白作为该病毒基因工程疫苗的成分提供了一定的理论基础。

mmunogenecity and immunoprotection of recombinant Hantaan virus nucleocapsid protrein(NP)expressed in E.coli were studied.First,mice were immunized with the recombinant NP,the titer of antibody against Hantaan virus was determineted using indirect immunofluorescenceassay,it was1:12800 in 4 weeks after immunized.Then,the spleen cells and sera of immunizedmice were transferred to 3￣4 day-old mice in 24 hours after the mice infected with lethal dose ofHantaan virus.The protection rates of spleen cells were 10～38%,but the sera could not protectmice from death,at the same time, it also did not change the living time of mice with lethal Han-taan virus infection. The results indicate that NP ean induce the cell-mediated immune protectionbut cannot induce the antibody-mediated immune protection and the early death in Hantaan virusinfection mice.