白纹伊蚊和埃及伊蚊经卵传递登革病毒的研究

Transovarial Transmisson of Dengue Viruses in Aedes albopictus and Aedes aegypti Mosquitoes

白纹伊蚊和埃及伊蚊通过吸食病毒液或叮吸有病毒血症的小鸡血后，能感染登革１～４型病毒，并能在蚊体内增萌。对感染雌蚊子１和子２代幼虫、雌性或雄性成虫４５５９只，分１０１批进行了病毒检测。白纹伊蚊子１代的批阳性率：登革１型为１０％（１／１０），２型为２２．２２％（２／９），３型为３３．３３％（４／１２），４型为２８．９５％（１１／３８）；登革１～４型的最低子代感染率依次为０．２０％、０．７１％、０．７０％和０．６３％。子２代对登革４型的批阳性率和最低子代的感染率分别为３５．２９％（６／１７）和０．９３％。登革４型感染埃及伊蚊子１和子２代的最低子代感染率分别为０．６３％和０．６０％。实验表明，白纹伊蚊和埃及伊蚊能经卵传递登革病毒。在本病地方性疫区中，这两种蚊虫在维持登革病毒的自然循环中起重要作用。

edes albopictus and Ae. aegypti mosquitoes from maialand China were tested for their abil-ity to transovarial transmission of dengue(DEN) viruses. DEN1～4 viruses for experiment wereprototype dengue strains(Hawaii,New Guinea,H87 and H241)and Ban18 strain of DEN4 isolat-ed from Yunnan,China.Parental females of Ae. albopictus and Ae aegypti were orally infectedwith DEN1～4 viruses,and 4559 larvae and adult of F1 and F2 generations from infected motherswere divided into 10 pools for the detection of the viruses. The positive rates of pools from Ae.albopictus of F1 were 10%(1/10) for DEN1,22.22%(2/9) for DEN2,33.33%(4/12)forDEN3 and 28.95%(11/38)for DEN4.The minimum filial infection rates(MFIR) of Ae.al-bopictus of F1 were successively 0.20%,0.71%,0.70%and 0.63%for DEN1～4.The positiverates of pools and MFIR from Ae.albopictus of F2 were 35.29%(6/17)and 0.93% for DEN 4.The positive rates of pcols from Ae.aegypti of F1 and F2 were 60%(3/5)and 33.33%(3/9),and their MFIR were 0.63 % and 0.60% for DEN4,respectively.The experimental studiesshowed that iofected Ae.albopictus and Ae.aegypti females are capable of transovarially trans-mitting DEN1～4viruses to their offspring,and these two species of mosquitoes from mainlandChina have the potential role in the natural cycle of the maintenance of dengue viruses in endemicareas of the disease.