几种细胞因子对HSV─1感染单核巨噬细胞的影响

Effect of Cytokines on the Infection ofMonocyte-Macrophages with HSV-1

以ＨＳＶ─１接种人单核细胞系Ｕ＿（９３７）和小鼠腹腔巨噬细胞，并在接种前后分别以不同的细胞因子处理。经病毒滴定、免疫荧光试验检测病毒抗原及多聚酶链反应技术检测病毒基因，研究了细胞因子对ＨＳＶ─１感染单核巨噬细胞的影响。结果证实，ＴＮＦ─α５０ｕ／ｍＬ 、Ｍ─ＣＳＦ２００ｕ／ｍＬＩＦＮ─γ１０００ｕ／ｍＬ、ＩＦＮ－α１０００ｕ／ｍＬ均能增强单核巨噬细胞对ＨＳＶ─１的抗性，加速细胞对ＨＳＶ─１ＤＮＡ的清除，抑制病毒抗原的表达；并能拮抗ＬＰＳ对ＨＳＶ─１在单核巨噬细胞中表达的促进作用。

n vztro hunian monocyte-line U937 cells and mouse peritoneal macrophages were inoculatedwith HSV-1,which were persistently treated with difterent cytokines since several days beforeor after inoculation. The effect of these cytokines on the infection of monocyte-niacrophages withHSV -1 was studied by virus titration,indirect immunofluorescent test for viral antigen and poly-merase chaln reaction technique for viral DNA in infected cells.The results showed that TNF - α50 u/mL,M-CSF 200 u/mL,IFN -γ1000 u/mL and IFN - α 1000 u/mL all enhanced the re-sistance of monocyte - macrophages to HSV - 1 infection,accelerated the clearance of virus DNAand inhibited the expression of viral antigen in the cells,moreover,they were antergic to the en-hancement activity of LPS for the virus replication in monocyte macrophages.