摘要
ＥＢ病毒胸苷激酶基因的扩增陈尚武，陈瑞君，黄迪，朱振宇，马涧泉（中山医科大学生化教研室，广州５１００８９）（中山医科大学肿瘤研究所，广州５１００６０）关键词Ｅｐｓｔｅｉｎ－Ｂａｒｒ病毒，胸苷激酶，基因，聚合酶链反应鼻咽癌（ｎａｓｏｐｈａｒｙｎｇｅａｌ...
ancer Research Institute,Sun Yet-sen University of Medical Sciences,Guangzhou 510060)Based on Epstein-Barr virus(EBV)DNA(B95-8)genomesequence data and the struc-ture of BXLF1 open reading frame coding for EBV thymidine kinase(TK)a pair of PCR primers was designed and synthesized.Template DNA was extracted rapidly from B95-8cells harboring EBV by using guanidinium isothiocyanate (Gu SCN)and silicacourse(CS).A 1843bp DNA frag-ment containing intact EBV TK gene was amplified by polymerase chain reaction.The PCR prod-uct was verified by cleaving with Ncol.
出处
《中国病毒学》
CSCD
1996年第3期287-288,共2页
Virologica Sinica
基金
国家教委博士点基金
关键词
EB病毒
胸苷激酶
基因
扩增
Epstein-Barr virus,Thymidine kinase,Gene,Polymerase chain reaction
