人类疱疹病毒6型可诱生TNF－α

The Production of TNF-αInduced by HHV-6 in Vitro

用生物活性法和双抗体夹心桥联酶免疫吸附（ＥＬＩＳＡ）法检测了人类疱疹病素６型（ＨＨＶ－６）ＧＳ株和南京地方株ＣＮ５，８，１０感染的淋巴细胞培养上清中的肿瘤坏死因子（ＴＮＦ）的水平，发现培养２４ｈ即可检出高水平的ＴＮＦ，４８～７２ｈ述到峰值，此后逐渐下降，与未感染耐照组比较有及其显著的差异（Ｐ＜０．００１）。ＧＳ株与地方株同诱生ＴＮＦ水平无儿著性差异（Ｐ＞０．１），三株地方株诱生ＴＮＦ也无显著性差异（Ｐ＞０．０５）。ＴＮＦ－α单抗可以完全中和培养上清中ＴＮＦ的活性，证实上清中有ＴＮＦ－α。与ＬＰＳ比较，ＨＨＶ－６诱生ＴＮＦ－α的能力要强得多。

here are high levels of TNF-α in the supernatants of lymphocyte culture infected with HHV-6 GS strain and the Nanjing loeal strains.TNF-α was detected by two methods:biological ac-tivity assay using L929 cells and ELISA.HHV-6 can induce TNF-α with in 24 h,maximal re-lease of TNF-α occurred during 48 h to 72 h post infection and then gradually decreased.The levelof TNF-α in infected culture suprenatants is far higher than that of uninfecter cells(P<0.001).In this ease,there is almost no difference between local strain and strain GS(P>0.1).Three lo-cal strains have same result.Specific monoclonal antibody against TNF-α can completely neutralizethe activity of TNF in the supernatants of these cells infected with HHV-6.The levels of TNF-αinduced by HHV-6 is much higher than that induced by LPS as a positive control.