风疹病毒分离株在BHK_（21）细胞中的形态与形态发生

Morphology and Morphogenesis of the Isolated Strain of Rubella Virus in BHK21 Cell

利用超薄切片电子显微镜技术对本室分离的风疹病病毒（ＲＶ）ＪＲ＿（２３）株在ＢＨＫ＿（２１），细胞中的形态及形态发生过程进行了研究，同时与ＲＶ标准野毒株Ｇｏｓ－１０作了比较。结果表明，ＲＶＪＲ＿（２３）株感染ＢＨＫ＿（２１），细胞后６ｈ开始于细胞浆内观察到病毒颗粒，９６ｈ达到高峰。病毒颗粒呈图形，有双层脂质包膜包绕，直径４５～７５ｎｍ，核衣壳２５～３５ｎｍ。细胞浆内见到大量病毒相关颗粒，直径２０～３０ｎｍ。病毒包膜来自于细胞浆中的空泡膜或细胞膜。被ＲＶ感染的细胞浆中还观察到“繁殖复合体”，由膜性结构包绕着许多类似病毒颗粒的囊泡构成。丙株ＲＶ在形态与形态发生方面未发现差异。

ltrathin secction electron microscopy was used to investigate the morphology and morpho-genesis of rubella virus(RV)JR23 and Gos-10 strains in BHK21 cells and its effects on cellularultrastructures.The results showed that virions were observed in the cytoplasm at 6 h postinfec-tion and a large number of enveloped virions were seen at 96 h postinfection.The virion wasspherical,45 to 75 nm in diameter,with a 25￣35 nm electron-dense core covered by a double-layered lcose envelope.A numbers of roughly spherieal electron-dense particles,20￣30 nm indiameter,could be seen in the cytoplasm.The virus was enveloped by budding through cytoplas-mic or vacuole membrane.There was no difference between the two strains in the morphologyand morphogenesis.