大鼠完整牙胚肾被膜下种植实验研究

Study on implanting rat tooth germs in renal capsule

目的:评价肾被膜作为组织工程牙根和牙周组织的种植环境。方法:分离出生后6 d SD大鼠下颌第一磨牙完整牙胚,种植于成年SD大鼠肾被膜下,4周后收获种植牙胚,组织学观察种植牙胚的牙根和牙周组织发育情况。结果:20个种植牙胚中,4个种植牙胚有牙根和牙周组织生成,剩余种植牙胚仅见牙本质继续发育。结论:肾被膜是组织工程牙根和牙周组织的适宜种植环境。

Objective： To evaluate whether the renal capsule is a suitable implanting place for tissue engineering of tooth mot and periodontal tissue. Method ：Mandibular first molar germs of postnatal 6 days SD rat were separated and implanted in renal capsules of adult SD rat. After 4 weeks, the implanted germs were harvested and development condition of tooth mot and periodontal tissue of the implanted germs was analysed histologically, Result： Development of tooth mot and periodontal tissue was observed in 4 of 20 implanted germs. In the rest implanted germs, extense growth of dentine was found. Conclusion：Renal capsule is a suitable implanting place for tissue engineering of tooth mot and periodontal tissue.