摘要
We investigated the radical scavenging effect and antioxidation property of polypeptide ex- tracted from Chlamys farreri (PCF) in vitro using chemiluminescence and electron spin resonance (ESR) methods. We examined the scavenging effects of PCF on superoxide anions ( O?2 ), hydroxyl radicals (OH·), peroxynitrite (ONOO-) and the inhibiting capacity of PCF on peroxidation of linoleic acid. Our experiment suggested that PCF could scavenge oxygen free radicals including superoxide anions ( O2? ) (IC50 =0.3 mg/ml), hydroxyl radicals (OH·) (IC50 = 0.2 μg/ml) generated from the reaction systems and effectively inhibit the oxidative activity of ONOO- (IC50 = 0.2 mg/ml). At 1.25 mg/ml of PCF, the inhibi- tion ratio on lipid peroxidation of linoleic acid was 43 %. The scavenging effect of PCF on O?2 , OH· and ONOO- free radicals were stronger than those of vitamin C but less on lipid peroxidation of linoleic acid. Thus PCF could scavenge free radicals and inhibit the peroxidation of linoleic acid in vitro. It is an anti- oxidant from marine products and potential for industrial production in future.
We investigated the radical scavenging effect and antioxidation property of polypeptide extracted from Chlamysfarreri （PCF） in vitro using chemiluminescence and electron spin resonance （ESR） methods. We examined the scavenging effects of PCF on superoxide anions （O2^-）, hydroxyl radicals （OH·）, peroxynitrite （ONOO^-） and the inhibiting capacity of PCF on peroxidation of linoleic acid. Our experiment suggested that PCF could scavenge oxygen free radicals including superoxide anions （O2^-） （IC50 =0.3 mg/ml）, hydroxyl radicals （OH·） （IC50 = 0.2 μg/ml） generated from the reaction systems and effectively inhibit the oxidative activity of ONOO^- （IC50 = 0.2 mg/ml）. At 1.25 mg/ml of PCF, the inhibition ratio on lipid peroxidation of linoleic acid was 43 %. The scavenging effect of PCF on O2^-, OH· and ONOO free radicals were stronger than those of vitamin C but less on lipid peroxidation of linoleic acid. Thus PCF could scavenge free radicals and inhibit the peroxidation of linoleic acid in vitro. It is an antioxidant from marine products and potential for industrial production in future.
基金
Supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology Bureau of Qingdao (No. 2001-28-50).
