RT-PCR法检测庚型肝炎病毒C(GBV-C)RNA

Detection of GB Virus C(GBV-C)RNA by RT-PCR

应用RT－PCR法在非甲～非戊型肝炎患者血清及单采浆站供血员血浆中检测到GBV－CRNA，扩增产物经序列测定进一步确证，在30例非甲～非戊型肝炎患者中阳性率为6．6％；在600例单采浆供血者血浆中阳性率为0．33％。提示GBV－C可能不是非甲～非戊型肝炎的主要致病因子，可能存在GBV－C健康携带者。

GBV-C RNA were detected out in the serum of patients with non-A, non - B, non -C, non-D and non -E hepatitis were detected by using RT-PCR. The amplified products were further verified by sequence analysis. The detectablerates in 30 patients and 600 doncrs were6. 6 % and 0. 33%, respectively. It could be speculated that GBV-C might not be the major pathogenic factor for non-A to non-E hepatitis, and a proportion of cases might be asymptomatic carriers.