人白细胞介素-2单克隆抗体的研究

Study on McAb to IL-2

应用杂交瘤技术获得6株抗人白细胞介素-2单克隆抗体细胞株，并对其特性进行分析，各单抗滴度不一，有的高达10(16)，有的则在1∶10左右，大多数（5种）为IgG型，少数（1种）为IgM型。单克隆抗体与纯化的IL－2有明显的特异性吸附（P＜0．01）。有4种单克隆抗体有相同的吸附位点，另外2种至少有3个吸附位点，位阻表现不一。

Six cell strains secreting McAbs to IL-2 were obtained by hybridoma tech-nique and their characteristics were investigated. The titers of McAbs secreted by the 6 strains varied from 1∶10 to 10-5. The McAbs secreted by 5 of the 6 strains were IgG, and that secreted by the rest one was IgM. Significant and specific adsorption was found between the McAbs and purified IL-2 (p＜0．01). The McAbs secreted by 4 of the 6 strains have thesame absorption sites, but those secreted by the other 2 strains have at least 3 absorption sites with inconsistent steric hindrance.