应用RP-HPLC分析基因工程人表皮生长因子肽谱

Peptide Mapping of Recombinant Human Epidermal Growth Factor by RP-HPLC

用DTT还原rhEGF中的二硫键，并用碘乙酰胺封闭巯基，用TPCK处理的胰蛋白酶消化rhEGF，经RP－HPLC分析，发现不同工程菌生产的rhEGF存在结构的细微差别。该法具有灵敏度高、重现性好等优点，适用于基因工程产品肽谱的常规检定。

After the disulfide bond in rhEGF was reduced by DTT and the sulfydryl was blocked by idoacetanide, it was digested by trypsase treated with TPCK and analyzed by RP -HPLC. The minute difference among the structures of rhEGF expressed in various engi-neered bacterial strains was found. The method showed good sensitivity and repetitivity, andwas suitable for the routine peptide mapping of genetic engineered products.