摘要
用DTT还原rhEGF中的二硫键,并用碘乙酰胺封闭巯基,用TPCK处理的胰蛋白酶消化rhEGF,经RP-HPLC分析,发现不同工程菌生产的rhEGF存在结构的细微差别。该法具有灵敏度高、重现性好等优点,适用于基因工程产品肽谱的常规检定。
After the disulfide bond in rhEGF was reduced by DTT and the sulfydryl was blocked by idoacetanide, it was digested by trypsase treated with TPCK and analyzed by RP -HPLC. The minute difference among the structures of rhEGF expressed in various engi-neered bacterial strains was found. The method showed good sensitivity and repetitivity, andwas suitable for the routine peptide mapping of genetic engineered products.
出处
《中国生物制品学杂志》
CAS
CSCD
1996年第3期129-131,共3页
Chinese Journal of Biologicals
关键词
重组
人表皮生长因子
肽谱
基因工程
研制
Recombinant human epidermal growth factor RP-HPLC EGF peptide map