胰岛素蛋白质工程：高活性［B10Asp］人胰岛素

Protein Engineering of Insulin: a High Potential[B10Asp] Human Insulin

用基因定位突变方法将胰岛素Ｂ链第１０位的Ｈｉｓ变为Ａｓｐ，获得高活力胰岛素──［Ｂ１０Ａｓｐ］人胰岛素。其受体结合能力和离体生物活力分别为猪胰岛素的２６２％和２３５％；体内生物活力也明显高于猪胰岛素；它的促细胞生长能力为猪胰岛素的１７４％。

B10His of insulin B chain was substituted with Asp by the means of site-directed mutagenesis.A high potent human insulin──[B10Asp] insulin was obtained. Its binding capacity to the insulin receptor and in vitro biological activity are 262% and 235% respectivaly as those of porcine insulin;the in vivo biological activity of [B10Aspj] human insulin is also higher than that of porcine insulin. The cell growth promoting activity of [B10Asp] human insulin is 174% as that of porcine insulin.