人胎肝造血干细胞增殖刺激物的分离及鉴定

Purification and Identification of A Hematopoietic Stem Cell Proliferation Stimulator From Human Fetal Liver

经过超滤、ＤＥＡＥ－Ｓｅｐｈａｃｅｌ、ＳｅｐｈａｃｒｙｌＳ－２００和Ｓｕｐｅｒｏｓｅ１２ＨＲ多步分离纯化，从人胎肝细胞原代培养上清中分离到一分子量为３５ｋＤ的单一活性组分，具有造血干细胞增殖刺激活性，定义为ＦＬＳ－４。ＦＬＳ－４可能是一种新型造血干细胞增殖刺激因子，与具有这类活性的ＩＬ－３、ＩＬ－６、ＧＭ－ＣＳＦ、ＦＬＴ３配基和ＳＣＦ等在理化特性或生物学性质上均有所差异，在胎肝造血活跃时期，是启动早期造血干细胞从Ｇ＿０期进入Ｓ期的主要候选活性物质。

Through several steps including ultrafiltration,chromatography on DEAE-Sephacel,Sephacryl S-200 and HPLC-Superose 12 HR,a substance of 35 kD termed as FLS-4 was isolated from human fetal livers of 3-5 months with highly activity of stimulating hematopoictic stem cell proliferation. In physical and biological nature,FLS-4 exhibited a unique character different from IL-3, IL-6, CM-CSF and FLT3 ligand which are known to have hematopoietic stem cell proliferation stimulating activity of different extent. FLS-4 is very likely to be a novel hematopoietic stem cell proliferation stimulator. During the period of active hematopoiesis in fetal liver , FLS4 might be the major candidagte in triggering hematopoietic stem cells from resting G0 into cycling phase.