利用原生质体诱变筛选碱性蛋白酶高产菌株

SCREENING FOR HIGH YIELD ALKALI NE PROTEINASE PRODUCING STRAIN BY UV TREATMENT OF PROTOPLASTS

本实验以枯草杆菌ＡＸ—４６为出发菌株，在原生质体形成及再生的最佳条件下制备原生质体，并对原生质体进行紫外诱变处理，对大量的再生突变株进行发酵筛选，获得高产菌株ＡＰ—１２，碱性蛋白酶产量由原来的３２００．８ｕ／ｍｌ提高到４３５３．１ｕ／ｍｌ，提高率达３６％。同时又对ＡＰ—１２菌株进行遗传稳定性考查，考查结果，ＡＰ—１２是高产稳定菌株。

In this study, Bacillus subtilis AX-46 was used as the initial organism. Under the optimum conditions of formation and regeneration of protoplasts, the protoplasts of Bacillus subtilis AX-46 were prepared.The high yield stable strain AP-12 were obtained after UV irradiation of protoplasts and fermentation screening of many regenerative mutants.The production of alkaline proteinase was increased by 36% from 3200. 8 to 4353. 1u/ml. Investigation of its genetic stability confirms that AP12 is a stable strain.