聚合酶链反应检测沅江县牛尿中的钩端螺旋体DNA

Detection of Leptospira Interrogans in Cattle Urinary Samples from Hunan by Polymerase Chain Reaction

本研究应用聚合酶链反应（ＰＣＲ）产物的凝胶电泳及Ｓｏｕｔｈｅｒｎ－ｂｌｏｔｔｉｎｇ，检测湖南４３份牛尿标本中的钩端螺旋体ＤＮＡ，前法阳性率为９．３％，后法为１４％，表明牛群具有较高的带菌率，是钩端螺旋体的主要宿主，是钩端螺旋体病的重要传染源。

cattle urinary samples from Hunan province was detected for leptospire by agarose gel electrophoresis and southern-blotting of polymerase chain reaction products. 4 of 43 samples were positive by agrose gel electrophoresis of PCR products and 6 of 43 samples were positive by southern-blotting of PCR products.It showed that cattles were not only main hosts but also important source of infection, which carring rate and excreting rate all were very high.