摘要
研究了兔肌乳酸脱氢酶Ｍ４（ＬＤＨ）在十二胺丁酸盐（ＤＡＢ）－环己反烷胶束溶液中的催化活性。发现ＬＤＨ在ＤＡＢ反胶束中的催化转换数（Ｋｃａｔ）同水溶液中的相近。ＬＤＨ在ＤＡＢ反胶束中的活性随增溶水量的增加而增加，随ＤＡＢ浓度的增加而降低，文中还提出了ＬＤＨ在ＤＡＮ反胶束中的增溶方式。
The enzymatic activity of rabbit muscle lactic dehydrogenase (LDH) solubillized in reverse micelles formed by dodecylammonium butyrare (DAB) in cyclohexane has ho investigated. It was found that the activity of LDH increases with the increase of solubilized water content. At high water content in 0.1mol·1-1 DAN cyclohexane solution, the turnover number of LDH in reverse micelles was close to that in aqueous solution. The activity of LDH increased with the decrease of DAB conathon at constant wo (=[H2O]/[DAB]).
出处
《生物物理学报》
CAS
CSCD
北大核心
1996年第3期409-413,共5页
Acta Biophysica Sinica
关键词
乳酸脱氢酶
反胶束
十二胺丁酸盐
Recerse micelles
Lactic dehydrogenase
Dodecylammonium butyrate
Cyclohexane
