摘要
用傅里叶变换红外光谱仪分析了坐骨神经损伤后Ｌ４－６脊髓节段腹角区域红外光谱变化，观察结果表明，损伤后８小时至３天以及１０天损伤侧显示磷酸二酯在团ｖａｓＰＯ２－和ｖｓＰＯ２－的１２４０ｃｍ－１和１０８０ｃｍ－１谱线明显增强；而且损伤后１０天１０８０ｃｍ－１谱线增强幅度较大。提示损伤引发了神经组织核酸（ＤＮ和ＲＮＡ）合成的增加。显示酰胺Ⅰ（ａｍｉｄｅⅠ）的１６５２ｃｍ－１谱线于损伤后３天增强，而１天和１０天减弱，揭示损伤引起的神经元蛋白质含量和结构的变化是非常复杂的。
The chgnges of Infrared Spectar were studied by FT-IR (fournier transform infrared spectra) in rat ventral horns of spinal cord during nerve regeneration after crushing the right static nerve. The results showed that the bands aat 1240cm-1 and 1080cm-1, due to the symmetrical and symmetrical stretching vibrations of phosphodiester, incresed compared with the control during & days after injury and 10 days after it. It suggested that injury induced the increase of nucleic acids(DNA and RNA). The band at 1652cm-1, due to amide Ⅰ of proteins, strengthened 3 days after injury and reduced 1 and 10 days post it. It suggests that the changes of content and constructure of protcins in neurons by nerve injury are very complex.
出处
《生物物理学报》
CAS
CSCD
北大核心
1996年第3期441-444,共4页
Acta Biophysica Sinica
