摘要
血管紧张素(ANG)Ⅱ对新生大鼠无血清培养第二代心肌成纤维细胞(FB)具有剂量依赖性的促进增殖、DNA和RNA以及胶原合成的作用。ANGⅡ107mol/L浓度时,FB的[3H]胸腺嘧啶核苷(Thymidine),[3H]尿嘧啶核苷(Uridine),[3H]脯氨酸(Proline)掺入较对照组分别增加167%,58%,174%,此刺激作用可被特异性ANGⅡ受体拮抗剂阻断;粉防己碱预处理也可抑制ANGⅡ上述作用,且呈剂量依赖性。以上结果提示:ANGⅡ通过受体介导直接刺激心肌FB增殖和胶原合成,引起心肌纤维化;钙拮抗剂粉防己碱对ANGⅡ导致的心肌纤维化具有抑制作用。
Withangiotensin Ⅱ(ANGⅡ, 10 10 -10 5 mol/L), a dose dependent augmentation of proliferation, DNA, RNA and collagen synthesis in serum free cultured passageⅡneonatal rat cardiac fibroblasts (FB) was observed. Treatment of FB with 10 7 mol/L ANGⅡ for 24h caused a significant increase in thymidine, uridine, proline incorporation by 167%, 58%, 174%respectively, while these stimulatory effects were completely inhibited by specifc ANGⅡ receptor antagonist,[Sar 1,Thr 8]ANGⅡ(10 5 mol/L). Pretreatment of tetrandrine (Tet)in various concentrations(10 10 10 5 mol/L)could also block stimulatory effects of ANGⅡ. These data suggest that the proliferation and collagen synthesis in cardiac FB induced by ANGⅡ may be mediated by ANGⅡ receptor, and these stimulatory effects can be inhibited by the calcium channel blocker tetrandrine.
出处
《中国应用生理学杂志》
CAS
CSCD
1996年第3期198-202,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金