脑胶质瘤细胞系对细小病毒MVM和H1易感性的研究

Susceptibility of Glioblastoma Cells to Wild Type Parvoviruses MVM and H1

[目的]测定人胶质瘤细胞系A172,U87MG,U138,U373,和鼠胶质瘤细胞系GL261与MT539对细小病毒MVM和H1的易感性,并观察野生型病毒在肿瘤细胞内部繁殖情况。[方法]体外培养肿瘤细胞,分别用不同剂量的MVM和H1病毒感染GL261,MT539和A172,U87MG,U138,U373细胞1h,继续培养5d,每天进行活细胞计数。空斑形成试验测定感染病毒(MOI=0.1)5d后细胞内部及细胞培养上清中的病毒总量。[结果]与未感染细胞(对照)比较,U138,U373,GL261细胞在MOI2和5的病毒剂量感染后活细胞数目明显减少,U87细胞数基本维持不变,A172和MT539细胞数轻微增加。MOI=0.1的病毒剂量对细胞的作用与对照没有差异。空斑形成试验结果表明U373和GL261在MOI=0.1的病毒感染5d后细胞内和培养上清中总病毒量略高于初始病毒量,其它细胞的总病毒量均低于初始病毒量。[结论]不同胶质瘤对细小病毒的易感性不同,U138,U373,GL261细胞对细小病毒H1或MVM易感,U87细胞次之,A172和MT539细胞不敏感。U373和GL261细胞有弱的产生子病毒的能力。

[Purpose] To evaluate the susceptibility of human glioma cell lines （A172, U87MG, U138, U373） and murine glioma eell lines （GL261 and MT539） to wild type parvoviruses H1, MVM and the replieation ability of wild type virus in these tumor cells. [Methods]GL261, MT539, A172, U87MG, U138 and U373 eells were infected with H1 and MVM virus respeetively for 1 hour at different MOI and cultured for 5 days in vitro. The number of living eells were eounted every day. Total amount of virus in eells and supernatant were examined by plaque assay （MOI=0.1） at the fifth day after infection. [Results] After infection with MOI 2 and 5, cell number of U138, U373, GL261 decreased markedly, U87MG unchanged and A172 and MT539 inereased a litter in eomparison with uninfeeted eells. There was no differenee between the doses at MOI 0.1 and eontrol. The total amount of viruses measured at fifth day after infeetion was lower or similar to the amount of initial in most eell lines. U373 and GL261 samples showed a slight increase of virus compared with initial. [Conclusions ] Different cell lines show different susceptibility to infeetion. U138, U373 and GL261 are suseeptibility to parvovirus infeetion in vitro; U87, medium and A172, MT539 are not suseeptive. U373 and GL261 have weak potence of produeing progeny virions.