荭草药材的质量研究

Study on quality for Herba Polygoni Orientalis

目的：建立荭草药材的定性、定量检测方法。方法：采用TLC鉴定荭草药材中荭草素、异荭草素、原儿茶酸和没食子酸，HPLC测定荭草中荭草素、异荭草素的质量分数。结果：采用Diamonsil C18色谱柱，以乙腈-0．1％磷酸溶液（18：82）为流动相，可使异荭草素、荭草素达到基线分离，异荭草素进样量在0．075～0．90μg、荭草素在0．041～0．49μg呈良好的线性关系，异荭草素和荭草素的平均回收率分别为98．8％，99．5％，RSD分别为2．1％，1．7％（n=9）。结论：本方法可以作为荭草药材的定性、定量检测方法。

Objective： To establish the qualitative and quantitative detective methods of Herba Polygoni Orientalis. Method： Isorientin, orienfin, protocatechuic acid and gallic acid in Herba Polygoni Orientalis were identified by TLC. The contents of isorienfin and orientin in Herba Polygoni Orientalis were determined by HPLC. Result： Isorientin, orientin, protocatechuic acid and gallic acid could be identified by TLC. Isofientin and orientin were well separated with Diamonsil CIS column and acetonitrile-0.1% phosphoric acid （ 18： 82） as mobile phase. The linear range of isorientin was 0. 075-0. 90 μg. The average recovery of isorientin was 98.8 % and RSD was 2.1%. The linear range of orientin was 0.041-0.49μg. The average recovery of orientin was 98.8 % and RSD was 2.1%. Conclusion： The methods can be used for qualitative identification and quantitation determination of Herba Polygoni Orientalis.