HPLC测定双丹颗粒中丹酚酸B和芍药苷的含量

Determination of salvianolic acid B and paeoniflorin in Shuangdan granules by HPLC

目的：建立双丹颗粒中丹酚酸B和芍药苷的含量测定方法。方法：采用高效液相色谱法，Zorbax SB-C18色谱柱，流动相A0．05％的磷酸水溶液，B含0．05％磷酸的4％乙腈甲醇溶液，梯度洗脱，流速1mL·min^-1，柱温25℃，检测波长230nm。结果：丹酚酸B在0．710～22．7μg（r=0．9999），芍药苷在0．103～3．30μg（r=0．9999）有良好的线性关系，测定了3个批次双丹颗粒中丹酚酸B和芍药苷的含量，其中丹酚酸B的含量范围为6．46～11．6mg·g^-1，芍药苷的含量范围为1．44～1．78mg·g^-1。结论：该方法准确度好、重复性好、稳定可靠，可以作为双丹颗粒质量控制的方法。

Objective： To develop a method for the determination of salvianolic acid B and paeoniflorin in Shuangdan granules. Method： The chromatographic separation was performed on a Zorbax SB C18 column with a linear gradient elution of phosphoric acid-water （0.05：100） and phosphoric acid-acetonitrile-methanol （0.05：4：96） was used. The flow rate was 1 mL·min^-1 and column temperature was set at 25 ℃. The UV detection wavelength was set at 230 nm. Result： The linear ranger was of 0.710-22.7 μg for salvianolic acid B and 0.103-3.30 μg for paconiflorin. Three hatches of Shuangdan granules were analyzed, and the content of salvianolic acid B ranged from 6.46- 11.6 mg·g^-1 and paconiflorin ranged from 1.44-1.78 mg·g^-1. Conclusion： The method was accurate, reproducible, reliable, and could be used for quantitative control of Shuangdan granules.